Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay

20091,051 citationsJournal Article

Authors

Andrew M. Kropinski · Public Health Agency of Canada

Erika J. Lingohr · Public Health Agency of Canada

Roger P. Johnson · Public Health Agency of Canada

Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

Topics & Keywords

Bacteriophages and microbial interactions RNA and protein synthesis mechanisms Monoclonal and Polyclonal Antibodies Research

UN Sustainable Development Goals

Zero hunger

Publication Details

Published in: Methods in molecular biology

Volume 501, pp. 69-76

DOI: 10.1007/978-1-60327-164-6_7

Field-Weighted Citation Impact: 11.91