Using QIIME to Analyze 16S rRNA Gene Sequences from Microbial Communities

2011747 citationsJournal Article

Authors

Justin Kuczynski · University of Colorado Boulder

Jesse Stombaugh · University of Colorado Boulder

William A. Walters · University of Colorado Boulder

Antonio González · University of Colorado Boulder

J. Gregory Caporaso · Northern Arizona University

Rob Knight · University of Colorado Boulder

Abstract

QIIME (canonically pronounced "chime") is a software application that performs microbial community analysis. It is an acronym for Quantitative Insights Into Microbial Ecology, and has been used to analyze and interpret nucleic acid sequence data from fungal, viral, bacterial, and archaeal communities. The following protocols describe how to install QIIME on a single computer and use it to analyze microbial 16S sequence data from nine distinct microbial communities.

Topics & Keywords

Microbial Community Ecology and Physiology Bacteriophages and microbial interactions Genomics and Phylogenetic Studies

UN Sustainable Development Goals

Life in Land

Publication Details

Published in: Current Protocols in Bioinformatics

Volume 36, Issue 1, pp. 10.7.1-10.7.20

DOI: 10.1002/0471250953.bi1007s36

Field-Weighted Citation Impact: 9.91