A real-time PCR assay for the differentiation of Candida species

201035 citationsJournal Article

Authors

Stephan Fricke · Leipzig University

Christian Fricke

Christoph Schimmelpfennig · Fraunhofer Institute for Cell Therapy and Immunology

Christopher Oelkrug · Fraunhofer Institute for Cell Therapy and Immunology

Uta Schönfelder · Ev. Diakonissenkrankenhaus Leipzig

R Blatz · Leipzig University

C. Zilch · Fraunhofer Institute for Cell Therapy and Immunology

Abstract

We carried out initial steps in validation of a PCR assay for the detection and differentiation of medically relevant Candida species. The PCR was improved by generating PCR standards, additional generation of melting curves for species identification and the possibility to investigate different specimens simultaneously.

Topics & Keywords

Antifungal resistance and susceptibility Fungal Infections and Studies Bacterial Identification and Susceptibility Testing

UN Sustainable Development Goals

Life in Land

Publication Details

Published in: Journal of Applied Microbiology

Volume 109, Issue 4, pp. 1150-1158

DOI: 10.1111/j.1365-2672.2010.04736.x

Field-Weighted Citation Impact: 1.37