Next‐generation sequencing: proof of concept for antenatal prediction of the fetal <scp>K</scp>ell blood group phenotype from cell‐free fetal <scp>DNA</scp> in maternal plasma

This method requires only one primer pair, and the large amount of sequence information obtained allows well for statistical analysis of the data. This general approach can be integrated into current laboratory practice and has numerous applications. Besides DNA-based predictions of blood group phenotypes, platelet phenotypes, or sickle cell anemia, and the determination of zygosity, various conditions of chimerism could also be examined using this approach. To our knowledge, this is the first report focused on antenatal blood group determination using NGS.