Phasing amplicon sequencing on Illumina Miseq for robust environmental microbial community analysis

2015303 citationsJournal Articlegold Open Access

Authors

Liyou Wu · University of Oklahoma

Chongqing Wen · University of Oklahoma

Yujia Qin · University of Oklahoma

Huaqun Yin · Central South University

Qichao Tu · University of Oklahoma

Joy D. Van Nostrand · University of Oklahoma

Tong Yuan · University of Oklahoma

Abstract

The developed strategy is robust for 16S rRNA gene amplicon sequencing. In addition, a similar strategy could also be used for sequencing other genes important to ecosystem functional processes.

Topics & Keywords

Microbial Community Ecology and Physiology Genomics and Phylogenetic Studies Environmental DNA in Biodiversity Studies

UN Sustainable Development Goals

Life in Land

Publication Details

Published in: BMC Microbiology

Volume 15, Issue 1, pp. 125-125

DOI: 10.1186/s12866-015-0450-4

Field-Weighted Citation Impact: 25.56