An Enzymatic Method for Glucose Determination in Body Fluids

Abstract 1. Details are given for the qualitative detection of glucose in urine and for its quantitative determination in urine and blood filtrates by an enzymatic procedure. 2. The method, which is highly specific, is based upon the release of hydrogen peroxide from glucose during its oxidation by glucose oxidase. The peroxide is detected by a suitable indicator in the presence of horseradish root peroxidase. 3. The results yielded by the new procedures have been compared with those obtained by other accepted methods.