Conventional transmission electron microscopy

2014245 citationsJournal Article

Authors

Mark Winey · University of Colorado Boulder

Janet B. Meehl · University of Colorado Boulder

Eileen O’Toole · University of Colorado Boulder

Thomas H. Giddings · University of Colorado Boulder

Abstract

Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during experimental design. Advanced electron microscopy techniques are listed as well. Finally, we point potential new users of TEM to resources to help launch their project.

Topics & Keywords

Advanced Electron Microscopy Techniques and Applications Nuclear Structure and Function RNA Research and Splicing

UN Sustainable Development Goals

Industry, innovation and infrastructure

Publication Details

Published in: Molecular Biology of the Cell

Volume 25, Issue 3, pp. 319-323

DOI: 10.1091/mbc.e12-12-0863

Field-Weighted Citation Impact: 7.89