Immunodetection of Glycosylated NT-proBNP Circulating in Human Blood

2008112 citationsJournal Article

Authors

Karina R Seferian · HyTest (Finland)

Natalia N Tamm · HyTest (Finland)

Alexander G Semenov · Lomonosov Moscow State University

Anastasia A Tolstaya

Ekaterina V Koshkina · Moscow City Hospital No 29

Mihail I Krasnoselsky

Alexander B. Postnikov · HyTest (Finland)

Daria V. Serebryanaya

Abstract

The central part of endogenous NT-proBNP is glycosylated, making it almost "invisible" for the antibodies specific to the mid-fragment of the molecule. Thus sandwich assays using even one antibody (poly- or monoclonal) specific to the central part of the molecule could underestimate the real concentration of endogenous NT-proBNP. MAbs specific to the N- and C-terminal parts of NT-proBNP (epitopes 13-24 and 63-76) are the best candidates to be used in an assay for optimal NT-proBNP immunodetection.

Topics & Keywords

Peptidase Inhibition and Analysis Heart Failure Treatment and Management Cardiac Structural Anomalies and Repair

UN Sustainable Development Goals

Good health and well-being

Publication Details

Published in: Clinical Chemistry

Volume 54, Issue 5, pp. 866-873

DOI: 10.1373/clinchem.2007.100040

Field-Weighted Citation Impact: 3.19