Directed Evolution To Investigate Steric Control of Enzymatic Oxidosqualene Cyclization. An Isoleucine-to-Valine Mutation in Cycloartenol Synthase Allows Lanosterol and Parkeol Biosynthesis

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDirected Evolution To Investigate Steric Control of Enzymatic Oxidosqualene Cyclization. An Isoleucine-to-Valine Mutation in Cycloartenol Synthase Allows Lanosterol and Parkeol BiosynthesisElizabeth A. Hart, Ling Hua, Lisa B. Darr, William K. Wilson, Jihai Pang, and Seiichi P. T. MatsudaView Author Information Department of Chemistry and Department of Biochemistry and Cell Biology Rice University, Houston, Texas 77005 Cite this: J. Am. Chem. Soc. 1999, 121, 42, 9887–9888Publication Date (Web):October 8, 1999Publication History Received21 July 1999Published online8 October 1999Published inissue 1 October 1999https://pubs.acs.org/doi/10.1021/ja992589bhttps://doi.org/10.1021/ja992589brapid-communicationACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views628Altmetric-Citations86LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Deprotonation,Fungi,Genetics,Peptides and proteins,Steroids Get e-Alerts