Reference Gene Selection for qRT-PCR Analysis in the Sweetpotato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

2013211 citationsJournal Articlegold Open Access

Authors

Rumei Li · Institute of Vegetables and Flowers

Wen Xie · Institute of Vegetables and Flowers

Shaoli Wang · Institute of Vegetables and Flowers

Qingjun Wu · Institute of Vegetables and Flowers

Nina Yang · Institute of Vegetables and Flowers

Xin Yang · Institute of Vegetables and Flowers

Huipeng Pan

Abstract

Our finding is the first step toward establishing a standardized quantitative real-time PCR procedure following the MIQE (Minimum Information for publication of Quantitative real time PCR Experiments) guideline in an agriculturally important insect pest, and provides a solid foundation for future RNA interference based functional study in B. tabaci.

Topics & Keywords

Molecular Biology Techniques and Applications Viral Infectious Diseases and Gene Expression in Insects Insect Resistance and Genetics

UN Sustainable Development Goals

Zero hunger

Publication Details

Published in: PLoS ONE

Volume 8, Issue 1, pp. e53006-e53006

DOI: 10.1371/journal.pone.0053006

Field-Weighted Citation Impact: 7.19