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Topographically, the Puna plateau of northwestern Argentina is the southern continuation of the Bolivian Altiplano. Its thickening and consecutive uplift result from the Andean orogeny. To better constrain the structural style and its progressive development, we have studied field data, topographic and satellite imagery, balanced cross sections, seismic reflection data, kinematic analysis of fault slip data, anisotropy of magnetic susceptibility (AMS), paleomagnetic data, and apatite fission track (AFT) data. Across the Puna plateau, Precambrian and Paleozoic basement ranges, bounded by high‐angle reverse faults (dips ≥ 60°), alternate with Cenozoic intermontane basins. Major thrusts trend NNE‐SSW and do not show a preferred vergence. Intermontane basins have various degrees of symmetry, depending on the geometries and attitudes of associated thrusts as well as on the magnitudes of their offsets. There is a close correlation between the surface expression of a basin and the amount of internal deformation. A line‐balanced cross section of the Puna at 25°S has yielded a Cenozoic shortening of 10–15%, in a direction subperpendicular to the orogen. By kinematic analysis of Cenozoic fault slip data we have obtained principal directions of strain rate across the Puna. Shortening axes are subhorizontal and trend on average WNW‐ESE (∼N110°), stretching axes are subvertical, and intermediate axes are subhorizontal and trend on average NNE‐SSW. Strain ellipsoids are dominantly of plane strain type, and they represent dip‐slip thrusting. From paleomagnetic and AMS data, shortening axes form a radial pattern around the eastern edge of the central Andes. The pattern is attributed to an inhomogeneous stress field, reflecting the eastward convex shape of the central Andean thrust front. From the history of burial and uplift, Andean shortening reached the northeastern part of the Puna in the late Eocene and the adjacent Eastern Cordillera in the late Eocene or early Oligocene. This shortening was presumably due to the Incaic phase of the Andean orogeny. In the eastern part of the orogen the onset of shortening was probably guided by preexisting Paleozoic and Mesozoic structures, so that Andean deformation propagated unevenly eastward.