DNA gyrase: an enzyme that introduces superhelical turns into DNA.

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Abstract

Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA.

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Bacteriophages and microbial interactions DNA and Nucleic Acid Chemistry

Publication Details

Published in: Proceedings of the National Academy of Sciences

Volume 73, Issue 11, pp. 3872-3876

DOI: 10.1073/pnas.73.11.3872

Field-Weighted Citation Impact: 43.23

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DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors

Abstract

Topics & Keywords

Publication Details