The Rhoptry Proteins ROP18 and ROP5 Mediate <em>Toxoplasma gondii</em> Evasion of the Murine, But Not the Human, Interferon-Gamma Response

<div><p>The obligate intracellular parasite <em>Toxoplasma gondii</em> secretes effector proteins into the host cell that manipulate the immune response allowing it to establish a chronic infection. Crosses between the types I, II and III strains, which are prevalent in North America and Europe, have identified several secreted effectors that determine strain differences in mouse virulence. The polymorphic rhoptry protein kinase ROP18 was recently shown to determine the difference in virulence between type I and III strains by phosphorylating and inactivating the interferon-γ (IFNγ)-induced immunity-related GTPases (IRGs) that promote killing by disrupting the parasitophorous vacuole membrane (PVM) in murine cells. The polymorphic pseudokinase ROP5 determines strain differences in virulence through an unknown mechanism. Here we report that ROP18 can only inhibit accumulation of the IRGs on the PVM of strains that also express virulent <em>ROP5</em> alleles. In contrast, specific <em>ROP5</em> alleles can reduce IRG coating even in the absence of <em>ROP18</em> expression and can directly interact with one or more IRGs. We further show that the allelic combination of <em>ROP18</em> and <em>ROP5</em> also determines IRG evasion and virulence of strains belonging to other lineages besides types I, II and III. However, neither ROP18 nor ROP5 markedly affect survival in IFNγ-activated human cells, which lack the multitude of IRGs present in murine cells. These findings suggest that ROP18 and ROP5 have specifically evolved to block the IRGs and are unlikely to have effects in species that do not have the IRG system, such as humans.</p> </div>