Characterisation of aurone biosynthesis in <i>Antirrhinum majus</i>

Aurones are bright yellow flavonoids produced in petals of a limited range of plant species, including Antirrhinum majus . The biosynthesis of aurones is thought to occur by the action of aureusidin synthase (AUS), and possibly aureusidin 7‐ O ‐glucosyltransferase (A7GT). The temporal and spatial occurrence of AUS and A7GT transcript was examined in wild‐type A. majus and two mutant lines; sulfurea , which has increased aurone production in petals, and violacea , which has reduced aurone production. AUS and A7GT transcript abundance was similar in all three lines, increasing during flower development coincident with yellow coloration. The spatial pattern of AUS occurrence was also similar in all three lines, being spatially restricted to the inner epidermis of the face and throat of the lower petal. A new recessive line ( CFR1011 ) with greatly reduced aurone production in all parts of the petal was identified by ethylmethanesulfonate mutagenesis of the homozygous recessive sulfurea line. Transcript abundance for AUS was not changed in the CFR1011 line compared with the wild‐type line, and neither were any point mutations detected in the coding sequences for AUS or A7GT. Thus, the sulfurea , violacea and CFR1011 mutations do not seem to control aurone production through a change in transcript abundance of the predicted biosynthetic genes AUS or A7GT. To examine AUS gene regulation further, the putative AUS gene promoter region was isolated and compared with other A. majus flavonoid gene promoters. A number of conserved potential regulatory regions were identified, in particular a consensus site for the MYB‐type transcription factors.