A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes

20091,082 citationsJournal Articlegold Open Access

Authors

Linda Amaral‐Zettler · Marine Biological Laboratory

Elizabeth A. McCliment · Marine Biological Laboratory

Hugh W. Ducklow · Marine Biological Laboratory

Susan M. Huse · Marine Biological Laboratory

Abstract

Massively parallel pyrosequencing of eukaryotic V9 hypervariable regions of SSU rRNA genes provides a means of estimating species richness from deeply-sampled populations and for discovering novel species from the environment.

Topics & Keywords

Bacteriophages and microbial interactions RNA modifications and cancer Parasitic Infections and Diagnostics

UN Sustainable Development Goals

Life below water

Publication Details

Published in: PLoS ONE

Volume 4, Issue 7, pp. e6372-e6372

DOI: 10.1371/journal.pone.0006372

Field-Weighted Citation Impact: 19.64