Tissue fractionation studies. 18. Resolution of mitochondrial fractions from rat liver into three distinct populations of cytoplasmic particles by means of density equilibration in various gradients

Beaufay, Bendall, Baudhuin, Wattiaux & de Duve (1959) described experiments in which mito- chondrial fractions from rat liver were partly re- solved into their components by density equilibra- tion in sucrose gradients, with either H20 or D20 as solvent. The results obtained in these investigations provided additional confirmation of the existence of lysosomes as a separate group of particles, distinct from mitochondria, and sug- gested further that urate oxidase, though almost.