Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains

2009177 citationsJournal Articlegold Open Access

Authors

David J. Lee · University of Birmingham

Lewis Bingle · University of Birmingham

Karin Heurlier · University of Nottingham

Mark J. Pallen · University of Birmingham

Charles W. Penn · University of Birmingham

Stephen Busby · University of Birmingham

Jon L. Hobman ·

Abstract

We have developed a counter-selective recombineering technique for epitope tagging or for deleting genes in E. coli. We have demonstrated the versatility of the technique by modifying the chromosome of the enterohaemorrhagic O157:H7 (EHEC), uropathogenic CFT073 (UPEC), enteroaggregative O42 (EAEC) and enterotoxigenic H10407 (ETEC) E. coli strains as well as in K-12 laboratory strains.

Topics & Keywords

CRISPR and Genetic Engineering Bacterial Genetics and Biotechnology Escherichia coli research studies

Publication Details

Published in: BMC Microbiology

Volume 9, Issue 1, pp. 252-252

DOI: 10.1186/1471-2180-9-252

Field-Weighted Citation Impact: 2.42

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Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains

Authors

Abstract

Topics & Keywords

Publication Details