Simple “On‐Demand” Production of Bioactive Natural Products

201596 citationsJournal Article

Authors

Edna Bode · Goethe University Frankfurt

Alexander O. Brachmann · Goethe University Frankfurt

Carsten Kegler · Goethe University Frankfurt

Rukayye Simsek · Goethe University Frankfurt

Christina Dauth · Goethe University Frankfurt

Qiuqin Zhou · Goethe University Frankfurt

Marcel Kaiser

Abstract

Exchange of the native promoter to the arabinose-inducible promoter PBAD was established in entomopathogenic bacteria to silence and/or activate gene clusters involved in natural product biosynthesis. This allowed the "on-demand" production of GameXPeptides, xenoamicins, and the blue pigment indigoidine. The gene clusters for the novel "mevalagmapeptides" and the highly toxic xenorhabdins were identified by this approach.

Topics & Keywords

Microbial Natural Products and Biosynthesis Insect Resistance and Genetics Biochemical and Structural Characterization

Publication Details

Published in: ChemBioChem

Volume 16, Issue 7, pp. 1115-1119

DOI: 10.1002/cbic.201500094

Field-Weighted Citation Impact: 8.83

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Simple “On‐Demand” Production of Bioactive Natural Products

Authors

Abstract

Topics & Keywords

Publication Details