Processing of Pro–Brain Natriuretic Peptide Is Suppressed by O-Glycosylation in the Region Close to the Cleavage Site

2009147 citationsJournal Articlebronze Open Access

Authors

Alexander G Semenov · HyTest (Finland)

Alexander B. Postnikov · HyTest (Finland)

Natalia N Tamm · HyTest (Finland)

Karina R Seferian · HyTest (Finland)

Н С Карпова · Lomonosov Moscow State University

Marina N Bloshchitsyna · HyTest (Finland)

Ekaterina V Koshkina ·

Abstract

Only proBNP that was not glycosylated in the region of the cleavage site could effectively be processed into BNP and NT-proBNP. Site-directed mutagenesis enabled us to ascertain the unique suppressing role of T71-bound O-glycan in proBNP processing.

Topics & Keywords

Heart Failure Treatment and Management Peptidase Inhibition and Analysis Transplantation: Methods and Outcomes

UN Sustainable Development Goals

Good health and well-being

Publication Details

Published in: Clinical Chemistry

Volume 55, Issue 3, pp. 489-498

DOI: 10.1373/clinchem.2008.113373

Field-Weighted Citation Impact: 8.00