Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor δ Chain

In the polymerase chain reaction (PCR), two specific oligonucleotide primers are used to amplify the sequences between them. However, this technique is not suitable for amplifying genes that encode molecules where the 5′ portion of the sequences of interest is not known, such as the T cell receptor (TCR) or immunoglobulins. Because of this limitation, a novel technique, anchored polymerase chain reaction (A-PCR), was devised that requires sequence specificity only on the 3′ end of the target fragment. It was used to analyze TCR δ chain mRNA's from human peripheral blood γδ T cells. Most of these cells had a Vδ gene segment not previously described (V δ 3), and the δ chain junctional sequences formed a discrete subpopulation compared with those previously reported.