Reversed-Phase, High-Pressure Liquid Chromatography of Peptides and Proteins with Ion-Pairing Reagents

1978191 citationsJournal Article

Authors

Milton T.W. Hearn · Health Research Council of New Zealand

Abstract

Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).

Topics & Keywords

Analytical Chemistry and Chromatography Mass Spectrometry Techniques and Applications Chemical Synthesis and Analysis

UN Sustainable Development Goals

Clean water and sanitation

Publication Details

Published in: Science

Volume 200, Issue 4346, pp. 1168-1170

DOI: 10.1126/science.206966

Field-Weighted Citation Impact: 22.54