Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX

20149 citationsJournal Article

Authors

Nasim Shahidi Hamedani · University of Bonn

Fabian Blümke · University of Bonn

Fabian Tölle · University of Bonn

Falk Rohrbach · University of Bonn

Heiko Rühl · Institute for Transfusion Medicine

Johannes Oldenburg · Institute for Transfusion Medicine

Günter Mayer ·

Abstract

Short biotinylated oligodeoxynucleotides immobilized on streptavidin-coated magnetic beads allow for convenient and rapid purification of single-stranded oligodeoxynucleotides from crude asymmetric PCR mixtures, facilitating the selection of DNA aptamers.

Topics & Keywords

Advanced biosensing and bioanalysis techniques Molecular Biology Techniques and Applications DNA and Nucleic Acid Chemistry

Publication Details

Published in: Chemical Communications

Volume 51, Issue 6, pp. 1135-1138

DOI: 10.1039/c4cc08233h

Field-Weighted Citation Impact: 0.28

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Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX

Authors

Abstract

Topics & Keywords

Publication Details