Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

20126,136 citationsJournal Articlegold Open Access

Authors

Jian Ye · National Institutes of Health

George Coulouris · National Center for Biotechnology Information

Irena Zaretskaya · National Institutes of Health

Ioana Cutcutache · Duke-NUS Medical School

Steve Rozen · Duke-NUS Medical School

Thomas Madden · National Center for Biotechnology Information

Abstract

We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the specificity threshold and other primer properties. This tool is publicly available at http://www.ncbi.nlm.nih.gov/tools/primer-blast.

Topics & Keywords

Molecular Biology Techniques and Applications Genomics and Phylogenetic Studies RNA and protein synthesis mechanisms

Publication Details

Published in: BMC Bioinformatics

Volume 13, Issue 1, pp. 134-134

DOI: 10.1186/1471-2105-13-134

Field-Weighted Citation Impact: 76.27

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Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

Authors

Abstract

Topics & Keywords

Publication Details