Green Fluorescent Protein as a Marker for Gene Expression

19946,739 citationsJournal Article

Authors

William W. Ward · Rutgers, The State University of New Jersey

Douglas C. Prasher · Woods Hole Oceanographic Institution

Abstract

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.

Topics & Keywords

Genetics, Aging, and Longevity in Model Organisms CRISPR and Genetic Engineering Animal Genetics and Reproduction

UN Sustainable Development Goals

Zero hunger

Publication Details

Published in: Science

Volume 263, Issue 5148, pp. 802-805

DOI: 10.1126/science.8303295

Field-Weighted Citation Impact: 32.89