The use of DAPI for identifying and counting aquatic microflora1

19805,070 citationsJournal Articlebronze Open Access

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Abstract

A highly specific and sensitive fluorescing DNA stain, 4′6‐diamidino‐2‐phenylindole (DAPI) was compared with acridine orange (AO) for counting aquatic microflora. Use of DAPI improved visualization and counting of <1‐ µ m bacteria and blue‐green algae in seston‐rich samples and extended sample storage to at least 24 weeks.

Topics & Keywords

Microbial Community Ecology and Physiology Molecular Biology Techniques and Applications Identification and Quantification in Food

Publication Details

Published in: Limnology and Oceanography

Volume 25, Issue 5, pp. 943-948

DOI: 10.4319/lo.1980.25.5.0943

Field-Weighted Citation Impact: 20.74

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The use of DAPI for identifying and counting aquatic microflora1

Authors

Abstract

Topics & Keywords

Publication Details