Multicolor Spectral Karyotyping of Human Chromosomes

19961,763 citationsJournal Article

Authors

Evelin Schröck · National Human Genome Research Institute

Stanislas du Manoir · National Human Genome Research Institute

Timothy Veldman · National Human Genome Research Institute

Brigitte Schoell · National Human Genome Research Institute

Johannes Wienberg · University of Cambridge

M.A. Ferguson‐Smith · University of Cambridge

Yi Ning

Abstract

The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.

Topics & Keywords

Genomic variations and chromosomal abnormalities Chromosomal and Genetic Variations Gene expression and cancer classification

Publication Details

Published in: Science

Volume 273, Issue 5274, pp. 494-497

DOI: 10.1126/science.273.5274.494

Field-Weighted Citation Impact: 42.61

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Multicolor Spectral Karyotyping of Human Chromosomes

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Abstract

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Publication Details