Plant Propagation Through Tissue Cultures

THE ORCHIDS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138 STAGES OF THE TISSUE CULTURE METHOD 138 Stage L Establishment of the Aseptic Culture . 139 Stage II: Multiplication of Propagula ......... , ........ ... . .. . ... . . ...... . . 140 Stage III: Preparation for Reestablishment of Plants in Soil . 140 REQUIREMENTS OF EACH STAGE ...... , . . .... ... . .. .... . .. ... . .. ... ..... . 140 The Explant of Stage I . 148 The Nutrient Medium . 150