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Abstract Cellulose acetate is one of the most important esters of cellulose. Depending on the way it has been processed cellulose acetate can be used for great varies of applications (e.g. for films, membranes or fibers). The properties of the applied cellulose acetates are very important for these applications. A special field for using cellulose acetate is the synthesis of porous, spherical particles, so called cellulose beads. Different types of technical cellulose acetates were used and their ability to form such cellulose beads was characterized. First the different types of cellulose acetates were characterized by means of solubility; turbidity and degree of substitution. In addition the molar mass and the distribution of substituents along the polymeric chain were analyzed. Next, the cellulose beads were synthesized within an emulsion process using these different cellulose acetates. Then the properties (particle size, porosity, morphology) of the cellulose beads were determined. Finally, the relationship between the characteristic of cellulose acetates and properties of cellulose beads was investigated.