Optical mapping as a routine tool for bacterial genome sequence finishing

200799 citationsJournal Articlegold Open Access

Authors

Phil Latreille · Monsanto (United States)

Stacie Norton · Monsanto (United States)

Barry S. Goldman · Monsanto (United States)

John Henkhaus · OpGen (United States)

Nancy Miller · Monsanto (United States)

W. Brad Barbazuk · Donald Danforth Plant Science Center

Helge B. Bode

Abstract

Our experience suggests that routine use of optical mapping in bacterial genome sequence finishing is warranted. When combined with data produced through 454 sequencing, an optical map can rapidly and inexpensively generate an ordered and oriented set of contigs to produce a nearly complete genome sequence assembly.

Topics & Keywords

Entomopathogenic Microorganisms in Pest Control Nematode management and characterization studies Insect symbiosis and bacterial influences

Publication Details

Published in: BMC Genomics

Volume 8, Issue 1, pp. 321-321

DOI: 10.1186/1471-2164-8-321

Field-Weighted Citation Impact: 6.01

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Optical mapping as a routine tool for bacterial genome sequence finishing

Authors

Abstract

Topics & Keywords

Publication Details