Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-{aminomethyl)-7-deazaguanosine

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Authors

Norihiro Okada · National Cancer Research Institute

Shigeru Noguchi · National Cancer Research Institute

Susumu Nishimura · Court of Appeal

Tadaaki Ohgi · Nagoya University

Toshio Goto · Nagoya University

P. F. Crain · University of Utah

James A. McCloskey ·

Abstract

A precursor of modified nucleoside Q isolated from E. coli methyl-deficient tRNA was determined to be 7-(aminomethyl)-7-deazaguanosine. The structure was deduced by means of its chromatographic and electrophoretic mobilities, and UV and mass spectra, in addition to comparison with the synthesized authentic compound. The same molecule is also found in tRNA of an E. coli mutant selected for deficient synthesis of modified nucleosides.

Topics & Keywords

RNA modifications and cancer Biochemical and Molecular Research DNA and Nucleic Acid Chemistry

Publication Details

Published in: Nucleic Acids Research

Volume 5, Issue 7, pp. 2289-2296

DOI: 10.1093/nar/5.7.2289

Field-Weighted Citation Impact: 2.03

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Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-{aminomethyl)-7-deazaguanosine

Authors

Abstract

Topics & Keywords

Publication Details