Target specificity of the CRISPR‐Cas9 system

2014362 citationsJournal Articlediamond Open Access

Authors

Xuebing Wu · Massachusetts Institute of Technology

Andrea J. Kriz · Massachusetts Institute of Technology

Phillip A. Sharp · Massachusetts Institute of Technology

Abstract

The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.

Topics & Keywords

CRISPR and Genetic Engineering Genetics, Aging, and Longevity in Model Organisms Pluripotent Stem Cells Research

Publication Details

Published in: Quantitative Biology

Volume 2, Issue 2, pp. 59-70

DOI: 10.1007/s40484-014-0030-x

Field-Weighted Citation Impact: 9.96

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Target specificity of the CRISPR‐Cas9 system

Authors

Abstract

Topics & Keywords

Publication Details