Mechanism and use of the commercially available viability stain, <i>Bac</i>Light

2004355 citationsJournal Article

Authors

Abstract

The mechanism is a combination of displacement of SYTO9 by PI and quenching of SYTO9 emissions by fluorescence resonance energy transfer. Confusing results can occur if the relative intensities of the stains or the concentration of PI relative to nucleic acid are not properly accounted for.

Topics & Keywords

RNA and protein synthesis mechanisms Glycosylation and Glycoproteins Research Bacterial Genetics and Biotechnology

UN Sustainable Development Goals

Affordable and clean energy

Publication Details

Published in: Cytometry Part A

Volume 61A, Issue 2, pp. 189-195

DOI: 10.1002/cyto.a.20069

Field-Weighted Citation Impact: 0.96