A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography

Abstract A sensitive and reliable RP‐HPLC method was developed using a C18 CLC‐ODS (M) – 4.6×250 mm 2 column and gradient elution for the analysis of phenolic compounds in propolis raw material and its products. A procedure for the extraction of phenolic compounds using aqueous ethanol (90%) with the addition of veratraldehyde as the internal standard (IS) was developed allowing to quantify ten compounds: caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin‐4′‐methyl ether (AME), isosakuranetin, drupanin, artepellin C, baccharin, and 2,2‐dimethyl‐6‐carboxyethenyl‐2 H ‐1‐benzopyran acid (DCBEN). The developed method gave good detection response and linearity in the range of 20.83–533.33 μg/mL.