Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents

19824,756 citationsJournal Article

Authors

Brian F. Meyer · Purdue University West Lafayette

N. R. Ferrigni · Purdue University West Lafayette

J. E. Putnam · Purdue University West Lafayette

Linda Jacobsen · Purdue University West Lafayette

D. E. NICHOLS · Purdue University West Lafayette

J. McLaughlin · Purdue University West Lafayette

Abstract

A method, utilizing brine shrimp (Artemia salina Leach), is proposed as a simple bioassay for natural product research. The procedure determines LC (50) values in microg/ml of active compounds and extracts in the brine medium. Activities of a broad range of known active compounds are manifested as toxicity to the shrimp. Screening results with seed extracts of 41 species of Euphorbiaceae were compared with 9KB and 9PS cytotoxicities. The method is rapid, reliable, inexpensive, and convenient as an in-house general bioassay tool.

Topics & Keywords

Environmental Toxicology and Ecotoxicology Cassava research and cyanide Bioactive Natural Diterpenoids Research

UN Sustainable Development Goals

Responsible consumption and production

Publication Details

Published in: Planta Medica

Volume 45, Issue 05, pp. 31-34

DOI: 10.1055/s-2007-971236

Field-Weighted Citation Impact: 2.75