Using protein microarray technology to screen anti-ERCC1 monoclonal antibodies for specificity and applications in pathology

201244 citationsJournal Articlegold Open Access

Authors

Donghui Ma · OriGene (United States)

Dror I. Baruch · OriGene (United States)

Youmin Shu · OriGene (United States)

Kehu Yuan · OriGene (United States)

Zairen Sun · OriGene (United States)

Kaiyan Ma · OriGene (United States)

T. N. Hoang ·

Abstract

In summary, the 8F1 clone is not suitable for ERCC1 IHC assay due to its cross-reactivity with PCYT1A protein. Two newly generated monoclonal antibodies, 4F9 and 2E12, demonstrated ultra-specificity against ERCC1 protein and superior performance for IHC analyses.

Topics & Keywords

Advanced Biosensing Techniques and Applications Monoclonal and Polyclonal Antibodies Research Gene expression and cancer classification

UN Sustainable Development Goals

Good health and well-being

Publication Details

Published in: BMC Biotechnology

Volume 12, Issue 1, pp. 88-88

DOI: 10.1186/1472-6750-12-88

Field-Weighted Citation Impact: 2.42