Rapid detection and serotyping of prunus necrotic ringspot virus in perennial crops by enzyme‐linked immunosorbent assay

SUMMARY Strains of prunus necrotic ringspot virus (NRSV) in hop and plum cultivars were rapidly and conveniently detected and serotyped by enzyme‐linked immunosorbent assay. Antisera produced against the G isolate of NRSV from cherry and the Paradise isolate of apple mosaic virus (ApMV) from apple were used concurrently to ensure detection of all serotypes and to differentiate between them. Both NRSV and ApMV serotypes occurred in plum either as single or mixed infections. By contrast, the NRSV serotype was not found in hop, which contained either ApMV or a serotype intermediate between NRSV and ApMV.