Effect of Overexpression of <i>Actinobacillus succinogenes</i> Phosphoenolpyruvate Carboxykinase on Succinate Production in <i>Escherichia coli</i>

Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc::kan, PEPCK overexpression increased succinate production 6.5-fold.