Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences

19771,253 citationsJournal Article

Authors

Axel Ullrich · University of California, San Francisco

John Shine · University of California, San Francisco

John M. Chirgwin · University of California, San Francisco

Raymond Pictet · University of California, San Francisco

Edmund Tischer · University of California, San Francisco

William J. Rutter · University of California, San Francisco

Howard M. Goodman

Abstract

Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3' terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.

Topics & Keywords

Pancreatic function and diabetes Diabetes and associated disorders Metabolism, Diabetes, and Cancer

Publication Details

Published in: Science

Volume 196, Issue 4296, pp. 1313-1319

DOI: 10.1126/science.325648

Field-Weighted Citation Impact: 154.24

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Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences

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Abstract

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Publication Details