A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus

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Authors

Alejandra Castillo Alvarez · Bioinstitut

Marion E. G. Brunck

Victoria Boyd · Commonwealth Scientific and Industrial Research Organisation

Richard Lai

Elena R. Virtue · Commonwealth Scientific and Industrial Research Organisation

Wenbin Chen · Queensland Health

Cheryl Bletchly · Queensland Health

Hans G. Heine

Abstract

Our one-step RT-PCR assay followed by sequencing is a rapid, accurate, and specific method for detection and subtyping of different neuraminidase subtypes from a range of host species and from different geographical locations.

Topics & Keywords

Influenza Virus Research Studies RNA and protein synthesis mechanisms Respiratory viral infections research

Publication Details

Published in: Virology Journal

Volume 5, Issue 1, pp. 77-77

DOI: 10.1186/1743-422x-5-77

Field-Weighted Citation Impact: 3.41

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A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus

Authors

Abstract

Topics & Keywords

Publication Details