Electrophoretic resolution of the ‘major outer membrane protein’ of <i>Escherichia coli</i> K12 into four bands

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Abstract

The cell envelope of Enterobacteriaceae consists of\ntwo membranes separated by a peptidoglycan layer.\nMethods have been developed to separate the cytoplasmic\nmembrane from the outer membrane.\nThe outer membrane of Escherichia coli contains 60%\nof the envelope protein. Using polyacrylamide\ngelelectrophoresis, Schnaitman found six protein\nbands in the outer membrane fraction, of which one\nband (mol. wt 44 000) accounted for 70% of the total\nouter membrane protein.

Topics & Keywords

Lipid Membrane Structure and Behavior Bacterial Genetics and Biotechnology RNA and protein synthesis mechanisms

Publication Details

Published in: FEBS Letters

Volume 58, Issue 1-2, pp. 254-258

DOI: 10.1016/0014-5793(75)80272-9

Field-Weighted Citation Impact: 21.58

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Electrophoretic resolution of the ‘major outer membrane protein’ of <i>Escherichia coli</i> K12 into four bands

Authors

Abstract

Topics & Keywords

Publication Details