Properties of the Reverse Transcription Reaction in mRNA Quantification

2004407 citationsJournal Articlebronze Open Access

Authors

Anders Ståhlberg · Tataa Biocenter (Sweden)

Joakim Håkansson · University of Gothenburg

Mikael Kubista · Tataa Biocenter (Sweden)

Abstract

RT-QPCR gene expression measurements are comparable only when the same priming strategy and reaction conditions are used in all experiments and the samples contain the same total amount of RNA. Experimental accuracy is improved by running samples in (at least) duplicate starting with the reverse transcription reaction.

Topics & Keywords

Molecular Biology Techniques and Applications RNA Research and Splicing Gene expression and cancer classification

Publication Details

Published in: Clinical Chemistry

Volume 50, Issue 3, pp. 509-515

DOI: 10.1373/clinchem.2003.026161

Field-Weighted Citation Impact: 8.64

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Properties of the Reverse Transcription Reaction in mRNA Quantification

Authors

Abstract

Topics & Keywords

Publication Details