Genotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small Amplicons

2004655 citationsJournal Articlebronze Open Access

Authors

Michael Liew · ARUP Laboratories (United States)

Robert J. Pryor · University of Utah

Robert Palais · University of Utah

Cindy Meadows · ARUP Laboratories (United States)

Maria Erali · ARUP Laboratories (United States)

Elaine Lyon · ARUP Laboratories (United States)

Carl T. Wittwer ·

Abstract

SNP genotyping by high-resolution melting analysis is simple, rapid, and inexpensive, requiring only PCR, a DNA dye, and melting instrumentation. The method is closed-tube, performed without probes or real-time PCR, and can be completed in less than 2 min after completion of PCR.

Topics & Keywords

Molecular Biology Techniques and Applications CRISPR and Genetic Engineering RNA Interference and Gene Delivery

Publication Details

Published in: Clinical Chemistry

Volume 50, Issue 7, pp. 1156-1164

DOI: 10.1373/clinchem.2004.032136

Field-Weighted Citation Impact: 6.71

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Genotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small Amplicons

Authors

Abstract

Topics & Keywords

Publication Details