Trypan Blue Exclusion Test of Cell Viability

20152,532 citationsJournal Article

Authors

Warren Strober · National Institute of Allergy and Infectious Diseases

Abstract

The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells).

Topics & Keywords

Cancer Research and Treatment Academic Writing and Publishing Genetics, Bioinformatics, and Biomedical Research

UN Sustainable Development Goals

Reduced inequalities

Publication Details

Published in: Current Protocols in Immunology

Volume 111, Issue 1, pp. A3.B.1-A3.B.3

DOI: 10.1002/0471142735.ima03bs111

Field-Weighted Citation Impact: 81.27