Comparability between insecticide resistance bioassays for mosquito vectors: time to review current methodology?

Even though the two assays can detect insecticide resistance, they may not be used interchangeably. While the diagnostic dose in the WHO susceptibility test does not allow for detecting shifts at low or extreme resistance levels, time-to-knockdown measured in the CDC bottle assay is a poor predictor of 24 h mortality. Therefore, dose-response assays could provide the most flexibility. New standardized bioassays are needed that produce consistent dose-response measurements with a minimal number of mosquitoes.