Genotyping of the Lactase-Phlorizin Hydrolase −13910 Polymorphism by LightCycler PCR and Implications for the Diagnosis of Lactose Intolerance

The new real-time PCR assay provides a rapid, labor-saving means for the genotyping of LPH C-->T(-13910). Use of the assay may assist in differentiating patients with primary hypolactasia from those with secondary hypolactasia and lactose intolerance, who may need further clinical examinations to diagnose their underlying primary diseases.