Analytical simulation of interfacial DNA hybridisation for design of an optimal nanotechnology handheld biosensor

DNA biosensors are becoming ubiquitous at current scale-level of nanotechnology; but still remain in research laboratory. The project objective is accessing feasibility for a commercial off-the-shelf optimally designed versatile handheld DNA biosensor. Therefore, thorough understandings of the principles behind design of DNA biosensor during biophysical interfacial transduction were explored. The Grahame equation was graphically solved at the electrolyte insulator interface of the DNA biosensor. Ion selective field-effect transistor (ISFET), integrated into nanotechnology, presents a suitable transduction platform to implement the benchmark protocol of DNA biosensors. Moreover, the identification or extraction of the optimal intrinsic and extrinsic parameters may in essence lead to stable, selective, sensitive, and a reliable optimally applicable DNA biosensor. This paper focuses on the simulation of essential mathematical and literature circuit equivalent models of interfacial DNA hybridisation. Model transfer functions were derived and the tools of control system theory were implemented for their usefulness to design an optimal nano-scaled DNA biosensor.