Generation and analysis of the improved human HAL9/10 antibody phage display libraries

2015130 citationsJournal Articlegold Open Access

Authors

Jonas Kügler · Technische Universität Braunschweig

Sonja Wilke · Science Factory

Doris Meier · Technische Universität Braunschweig

Florian Tomszak · Technische Universität Braunschweig

André Frenzel · Technische Universität Braunschweig

Thomas Schirrmann · Technische Universität Braunschweig

Stefan Dübel

Abstract

The highly diverse universal antibody gene libraries HAL9/10 were constructed using an optimized scFv phagemid vector design. Analysis of selected antibodies revealed that the complete amino acid diversity in the CDR-H3 was also found in selected scFvs showing the functionality of the naive CDR-H3 diversity.

Topics & Keywords

Monoclonal and Polyclonal Antibodies Research Glycosylation and Glycoproteins Research Advanced Biosensing Techniques and Applications

Publication Details

Published in: BMC Biotechnology

Volume 15, Issue 1, pp. 10-10

DOI: 10.1186/s12896-015-0125-0

Field-Weighted Citation Impact: 12.99

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Generation and analysis of the improved human HAL9/10 antibody phage display libraries

Authors

Abstract

Topics & Keywords

Publication Details