Accuracy and quality of massively parallel DNA pyrosequencing

20071,295 citationsJournal Articlegold Open Access

Authors

Julie A. Huber · Marine Biological Laboratory

Hilary G. Morrison · Marine Biological Laboratory

Mitchell L. Sogin · Marine Biological Laboratory

David B. Mark Welch · Marine Biological Laboratory

Abstract

By using objective criteria to eliminate low quality data, the quality of individual GS20 sequence reads in molecular ecological applications can surpass the accuracy of traditional capillary methods.

Topics & Keywords

Microbial Community Ecology and Physiology Protist diversity and phylogeny Environmental DNA in Biodiversity Studies

Publication Details

Published in: Genome biology

Volume 8, Issue 7, pp. R143-R143

DOI: 10.1186/gb-2007-8-7-r143

Field-Weighted Citation Impact: 72.73

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Accuracy and quality of massively parallel DNA pyrosequencing

Authors

Abstract

Topics & Keywords

Publication Details