Rapid Flow Cytometric Analysis of the Cell Cycle in Intact Plant Tissues

19831,949 citationsJournal Article

Authors

David W. Galbraith · University of Nebraska–Lincoln

Kristi R. Harkins · University of Nebraska–Lincoln

Joyce M. Maddox · University of Nebraska–Lincoln

Nicola M. Ayres · University of Nebraska–Lincoln

D.P. Sharma · University of Nebraska–Lincoln

E. Firoozabady · University of Nebraska–Lincoln

Abstract

Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.

Topics & Keywords

Plant Molecular Biology Research Tree Root and Stability Studies Plant responses to water stress

Publication Details

Published in: Science

Volume 220, Issue 4601, pp. 1049-1051

DOI: 10.1126/science.220.4601.1049

Field-Weighted Citation Impact: 18.06

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Rapid Flow Cytometric Analysis of the Cell Cycle in Intact Plant Tissues

Authors

Abstract

Topics & Keywords

Publication Details