A Modified Technique of Immunoelectrophoresis Facilitating the Identification of Specific Precipitin Arcs,

Summary A modification of the technique of immunoelectrophoresis has been developed which facilitates the identification of individual precipitin arcs in complex mixtures of proteins such as human serum. This modified procedure consists of the concurrent reaction of the antiserum with the electrophoretically separated primary antigen solution (e.g., whole serum) and with a known protein constituent. Examples are presented of the application of the technique to the identification of the components in Cohn Fraction IV-7 (primarily the iron-binding globulin), ceruloplasmin, and the urinary proteins in a case of myeloma.